Author Abstract – Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA(mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipitation assay followed by Northern blot analysis. We report that biotinylated miR-27 does not efficiently associate with Ago compared to unmodified miR-27. These results suggest that 3′-end biotin-modified miRNAs are questionable monitors of miRNA function in cells.
3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells.
- EBV Noncoding RNA Binds Nascent RNA to Drive Host PAX5 to Viral DNA
- High-Throughput RNA sequencing based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project
- Secondary Structure of a Conserved Domain in an Intron of Influenza A M1 mRNA
- Structural insights into the stabilization of MALAT1 noncoding RNA by a bipartite triple helix
EBER2 is an abundant nuclear noncoding RNA expressed by the Epstein-Barr virus (EBV). Probing its possible chromatin localization by CHART revealed EBER2’s presence at the…
High-Throughput RNA sequencing based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project
Using high-throughput RNA sequencing (RNA-seq) data from 50 common lymphoma cell culture models from the Cancer Cell Line Encyclopedia project, we performed an unbiased…
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Influenza A virus utiizes RNA throughout infection. Little is known, however, about the roles of RNA structures in these processes. A previous bioinformatics survey predicted…